Journal: STAR Protocols
Article Title: Protocol for spatial proteomic profiling of tonsil cancer microenvironments using multiplexed imaging-powered deep visual proteomics
doi: 10.1016/j.xpro.2025.103901
Figure Lengend Snippet: Sample preparation workflow for MACSima imaging system (A) Sample alignment guide showing the sectioning area compatible with the two-well imaging frame. (B) Guide for tissue sectioning onto PEN membrane slides with sections aligned to imaging frame wells. (C) Assembly of the two-well imaging frame, designed to secure tissue sections while enabling reagent access during automated staining cycles. (D) Deepwell plate (96-well format) preloaded with fluorescently conjugated antibodies for automated dispensing. (E) System rack setup, showing the imaging frame and deepwell plate. (F) Sample regions of interest (ROI) and autofocus configuration. The screenshots originate from the interface of MACSima Imaging System (#130-121-164 ) with software v.0.13.0.
Article Snippet: MACSima two-well imaging frame , Miltenyi Biotec , 130-124-675.
Techniques: Sample Prep, Imaging, Membrane, Staining, Software